Amelogenin Peptide-Chitosan Hydrogel for Biomimetic Enamel Regrowth

We designed synthetic peptides that have demonstrated an effective remineralization potential to restore incipient enamel decay. In order develop a clinically viable approach we incorporated the amelogenin-derived P26 and P32 into chitosan hydrogel examined their efficacy in of enamel. Peptides exhibited increased stability vitro as compared solution at room temperature 37°C. Tooth models for erosion (sections) white spot lesions (blocks) were subject periods demineralization. Treatment groups subjected artificial saliva presence (P26-CS P32-CS). Quantitative light-induced fluorescence (QLF) was employed analyze mineral density following demineralization across all treatment groups. Scanning electron microscopy nanoindentation used characterize surface structure mechanical strength regrown Control sections treated randomly distributed, tiny, needle-shaped crystals with low packing porosities displaying mineralization defects. samples P26-CS or P32-CS denser coating organized hydroxyapatite (HAP) formed covering entire surfaces demineralized window. The hardness modulus after no significant difference properties between two peptide hydrogels. Analysis by QLF showed alone significantly enhanced remineralization. blocks had better than P26-CS.